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Biotechnology - 11

1-	Virulence trait of Agrobacterium tumefaciens is borne on
	A -	chromosomal DNA
	B -	tumour inducing plasmid DNA
	C -	both chromosomal and plasmid DNA
	D -	cryptic plasmid DNA
2-	The size of the virulent plasmid of Agrobacterium tumefaciens is
	A -	40-80 kb
	B -	80-120 kb
	C -	140-235 kb
	D -	>235 kb
3-	Which of the following is not true about the helper plasmids?
	A -	These can replicate in Agrobacterium
	B -	These help in the mediating conjugation of intermediate vectors
	C -	These can't replicate in Agrobacterium
	D -	All of the above
4-	Which technique is used to introduce genes into dicots?
	A -	electroporation
	B -	particle acceleration
	C -	microinjection
	D -	Ti plasmid infection
5-	Direct DNA uptake by protoplasts can be stimulated by
	A -	polyethylene glycol (PEG)
	B -	decanal
	C -	luciferin
	D -	All of these
6-	In a plant tumour cell
	A -	complete Ti plasmid is incorporated in plant nuclear DNA
	B -	different parts of the Ti-plasmid are incorporated
	C -	only a small specific segment of callus T DNA is incorporated
	D -	may vary from plant to plant
7-	Co-integrating transformation vectors must include a region of homology in
	A -	the vector plasmid
	B -	the Ti-plasmid
	C -	between vector plasmid and Ti-plasmid
	D -	None of these
8-	Crown gall tissue
	A -	can be cultivated in vitro in absence of bacteria
	B -	retains tumorous properties when cultivated
	C -	Both (a) and (b)
	D -	shows tumorous properties only in presence of bacteria
9-	Integrated nopaline T-DNA occurs as
	A -	single segment
	B -	two segments
	C -	three segments
	D -	four segments
10-	Opines are
	A -	amino acid derivatives found in tumor tissues
	B -	amino acid derivatives found in normal tissues
	C -	amino acid derivatives found in both normal as well as tumor tissues
	D -	None of the above

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