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Biotechnology - 12

1-	Which of the following is true about Agrobacterium tumefaciens?
	A -	It causes crown gall disease of plants
	B -	It infects gymosperms
	C -	It infects dicotyledonous angiosperms
	D -	All of the above
2-	In the liposome mediated gene transfer in plants, nucleic acids are
	A -	protected from nuclease digestion
	B -	stable in liposomes
	C -	Both (a) and (b)
	D -	Not stable in liposomes
3-	Advantage of microprojectile method over microinjection method for gene transfer in plants include
	A -	intact cells are used
	B -	method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
	C -	gene can be transferred to many cells simultaneously
	D -	All of the above
4-	On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
	A -	12 bp
	B -	20 bp
	C -	25 bp
	D -	30 bp
5-	Agrobacterium tumefaciens is a
	A -	gram (+) bacteria
	B -	gram (-) bacteria
	C -	a fungi
	D -	a yeast
6-	Microprojectile method of gene transfer in plants involves delivery of DNA
	A -	with the help of micromanipulator
	B -	with the help of bolistics
	C -	with the help of needles
	D -	Any of the above
7-	Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
	A -	vir A and vir G
	B -	vir C and vir D
	C -	vir B and vir E
	D -	vir A and vir B
8-	Integrated octopine T DNA occurs as
	A -	single segment
	B -	two segments
	C -	three segments
	D -	four segments
9-	Liposomes mediated gene transfer in plants involves
	A -	plasmid DNA enclosed in a lipid bag
	B -	fusion of liposomes with protoplast
	C -	use of polyethylene glycol (PEG)
	D -	All of the above
10-	Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
	A -	Acetosyringone
	B -	α-hydroxy syringone
	C -	Both (a) and (b)
	D -	None of these

[Người đăng: Thành Lãm - ST]

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