Opines that are present in crown gall tumour include
A -
octopine
B -
nopaline
C -
agropine
D -
All of these
2-
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
A -
pRK 2013
B -
pRN 3
C -
Either (a) or (b)
D -
None of these
3-
Which of the following is true about T DNA?
A -
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B -
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
C -
Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D -
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
4-
Which of the following is not true about the direct repeats flanking T-DNA?
A -
They are conserved between nopaline and octopine Ti-plasmids
B -
These repeats are transferred intact to the plant genome
C -
These are important in integration mechanism
D -
All of the above
5-
The left segment of octopine T-DNA (TL) is necessary for
A -
enzymes for agropine biosynthesis
B -
tumour formation
C -
conjugative transfer
D -
All of these
6-
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
A -
It is carried out with the help of micromanipulator
B -
The recipient cells are immobilized on artificial support or artificially bound to substarate
C -
It employs needle with diameter greater than cell diameter
D -
All of the above
7-
The right segment of octopine T-DNA (TR) is necessary for
A -
enzymes for agropine biosynthesis
B -
tumour formation
C -
conjugative transfer
D -
All of these
8-
Opine synthesis is the property
A -
conferred to plant cells when it transformed by Agrobacterium tumefaciens
B -
determined by the bacteria Agrobacterium tumefaciens
C -
Both (a) and (b)
D -
of normal plant cells
9-
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
A -
one set of sequence necessary for gene transfer
B -
two sets of sequence necessary for gene transfer
C -
three sets of sequence necessary for gene transfer
D -
four sets of sequence necessary for gene transfer
10-
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as