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Biotechnology - 26

1-	Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
	A -	episomally only
	B -	integrated only
	C -	either episomally or integrated at high copy number
	D -	either episomally or integrated at low copy number
2-	Which of the following histone is not present in SV40 virus?
	A -	H1
	B -	H2A
	C -	H3
	D -	H4
3-	Autonomously replicating sequence is not present in
	A -	YIp
	B -	YRp
	C -	YCp
	D -	YAC
4-	In case of Baculoviruses, the foreign gene
	A -	is inserted downstream of polyhedrin promoter
	B -	is inserted downstream of viral origin of replication
	C -	is inserted between the polyhedrin promoter and downstream sequences
	D -	is inserted randomly in viral genome
5-	α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
	A -	His 3
	B -	Lys 2
	C -	Ura 3
	D -	Trp 1
6-	Yeast episomal plasmids (YEp) were constructed using
	A -	2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
	B -	2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
	C -	12μm yeast plasmid and E coli vector pMB 9
	D -	12μm yeast plasmid and E coli vector pBR 322
7-	Which of the following is/are commonly used promoter of S. cerevisiae?
	A -	Galactokinase (GAL 1)
	B -	Alcohol dehydrogenase II (ADH 2)
	C -	Phosphoglycerate kinase (PGK)
	D -	All of the above
8-	Maximum amount of foreign DNA introduced into SV 40 viruses is
	A -	10 kb
	B -	5 kb
	C -	2.5 kb
	D -	1 kb
9-	Recombinant SV40 viruses introduce the foreign DNA into cells
	A -	with the DNA mediated transfection
	B -	without the DNA mediated transfection
	C -	with the RNA mediated transfection
	D -	without the RNA mediated transfection
10-	Which one of the following is the most stable vector?
	A -	YEp
	B -	YRp
	C -	YAC
	D -	YIp

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