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Biotechnology - 27

1-	SV 40 vectors, in which early region replacements are carried out, require
	A -	COS cell line
	B -	SV 40 helper virus
	C -	pBR 322 vector
	D -	None of these
2-	Cloning genes in the yeast offer which of following advantage(s)?
	A -	Over production of proteins of commercial value
	B -	Ability to glycosylate proteins during secretion
	C -	Ability to clone large piece of DNA
	D -	All of the above
3-	Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
	A -	Allows transformation by using crossing over
	B -	Has a origin of replication and rep genes
	C -	Carries autonomously replicating sequence
	D -	Carries centromere and telomere sequences
4-	Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
	A -	Contains centromeric sequences
	B -	Segregates during meiosis and mitosis
	C -	Functions as true chromosome
	D -	All of the above
5-	Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
	A -	Vaccinia virus
	B -	Baculo virus
	C -	Papilloma virus
	D -	Retrovirus
6-	Polyhedrin protein is
	A -	non essential for baculo virus replication
	B -	major component of protein matrix in which viruses are embedded
	C -	Both (a) and (b)
	D -	essential for baculo virus replication
7-	Cos cell line is
	A -	cohesive end site of phage λ
	B -	derivative of permissive CV-1 monkey cell line
	C -	cohesive initial site of phage λ
	D -	derivative of non-permissive CV-1 monkey cell line
8-	Lytic infection of SV 40 does not occur in
	A -	permissive cells
	B -	non permissive cells
	C -	Both (a) and (b)
	D -	None of these
9-	Moloney-Mu-leukemia virus is an example of
	A -	Vaccinia virus
	B -	Baculo virus
	C -	Retro virus
	D -	None of these
10-	Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
	A -	Allows transformation by using crossing over
	B -	Has a origin of replication and rep genes
	C -	Carries autonomously replicating sequence
	D -	Carries centromere and telomere sequences

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