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Biotechnology - 28

1-	The size of the genome of SV 40 is
	A -	7.2 kb
	B -	69 kb
	C -	5.2 kb
	D -	110 kb
2-	YAC vectors can be used to obtain artificial chromosomes because it carries
	A -	centromere
	B -	telomere
	C -	autonomously replicating sequence
	D -	All of these
3-	Maximum insert size in case of adenovirus vectors is
	A -	6-8 kb
	B -	4-6 kb
	C -	10-12 kb
	D -	20 kb
4-	SV 40 vectors, in which late region replacements are done, require
	A -	COS cell line
	B -	SV 40 helper virus
	C -	pBR 322 vector
	D -	None of these
5-	Which of the following(s) is/are the most commonly used markers in yeast?
	A -	His 3
	B -	Ura 3
	C -	Trp 1
	D -	All of these
6-	Which of the following are produced in high copy number?
	A -	YEp
	B -	YRp
	C -	Both (a) and (b)
	D -	YCp
7-	5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is
	A -	His 3
	B -	Lys 2
	C -	Ura 3
	D -	Trp 1
8-	Which of the following promoters is repressed in presence of glucose?
	A -	Galactokinase (GAL 1)
	B -	Acid phosphatase (PHOS)
	C -	Both (a) and (b)
	D -	Phosphoglycerate kinase (PGK)
9-	Which of the following can be used for reducing the size of gene inserts containing introns?
	A -	Vaccinia virus
	B -	Baculo virus
	C -	Retro virus
	D -	None of these
10-	Bovine papilloma virus has a circular double stranded DNA genome of about
	A -	2.5 kb
	B -	5.2 kb
	C -	7.9 kb
	D -	9.5 kb

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