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Biotechnology - 39

1-	Maximum size of foreign DNA that can be inserted into a replacement vector is
	A -	25-30 kb
	B -	18-20 kb
	C -	20-25 kb
	D -	40-50 kb
2-	Which of the following is not true about phagemid?
	A -	Contain functional origin of replication of the plasmid and λ phage
	B -	May be propagated as a plasmid or as phage in appropriate strain
	C -	Contain λ att site
	D -	Can only be propagated as phage
3-	pBR 322 has/have which of the following selection marker(s)?
	A -	Amp^r
	B -	Tet^r
	C -	Kan^r
	D -	Both (a) and (b)
4-	A plasmid can be considered as a suitable cloning vector if
	A -	it can be readily isolated from the cells
	B -	it possesses a single restriction site for one or more restriction enzymes
	C -	insertion of foreign DNA does not alter its replication properties
	D -	All of the above
5-	Difference between λ gt 10 and λ gt 11 vectors is that
	A -	λ gt 11 is an expression vector
	B -	λ gt 10 is an expression vector
	C -	λ gt 10 is a replacement vector
	D -	λ gt 11 is a replacement vector
6-	λ ZAP vector is an example of
	A -	phage
	B -	phagemid
	C -	cosmid
	D -	plasmid
7-	λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
	A -	6-7 kb
	B -	1-2 kb
	C -	40-44 kb
	D -	20-23 kb
8-	Select the wrong statement about plasmids?
	A -	It is extrachromosomal
	B -	It is double stranded
	C -	Its replication depends upon host cell
	D -	It is closed and circular DNA
9-	Stuffer is
	A -	the right arm of the vector DNA
	B -	the left arm of the vector DNA
	C -	central fragment of the vector DNA
	D -	None of the above
10-	Conjugative plasmids
	A -	do not exhibit antibiotic resistance
	B -	carry transfer genes called the tra genes
	C -	exhibit antibiotic resistance
	D -	do not carry transfer genes

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