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Biotechnology - 41

1-	EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
	A -	6-7 kb
	B -	15-25 kb
	C -	40-44 kb
	D -	1-2 kb
2-	Size of the DNA that can be packaged into a λ phage is
	A -	50 kb
	B -	35-53 kb
	C -	40-50 kb
	D -	any size
3-	Charon 34 and Charon 35 are the examples of
	A -	plasmid vector
	B -	cosmid vector
	C -	phage vector
	D -	phagemid vector
4-	Cosmid vectors are used for
	A -	cloning small fragments of DNA
	B -	cloning large fragments of DNA
	C -	cloning prokaryotic DNA only
	D -	cloning eukaryotic DNA only
5-	Plasmids which are maintained as limited number of copies per cell are known as
	A -	stringent plasmids
	B -	relaxed plasmids
	C -	cryptic plasmids
	D -	All of these
6-	Cryptic plasmids
	A -	do not exhibit any phenotypic trait
	B -	exhibit many phenotypic traits
	C -	exhibit one phenotypic traits
	D -	exhibit antibiotic resistance
7-	Phagemid consist of
	A -	plasmid vector carrying λ phage's cos site
	B -	plasmid vector carrying λ attachment (λ att) site
	C -	plasmid vector carrying origin of replication of λ phage only
	D -	plasmid vector carrying origin of replication of plasmid only
8-	Maximum size of foreign DNA that can be inserted into an insertion vector is
	A -	35 kb
	B -	18 kb
	C -	50 kb
	D -	27 kb
9-	Plasmids which are maintained as multiple copy number per cell are known as
	A -	stringent plasmids
	B -	relaxed plasmids
	C -	cryptic plasmids
	D -	None of these
10-	Cosmid vectors are
	A -	plasmids that contain fragment of λ DNA including the cos site
	B -	phages that lack cos site
	C -	plasmids that have no selection marker
	D -	cryptic plasmids

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