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1-
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Which is/are the naturally occurring plant auxins?
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A -
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Indole acetic acid (IAA)
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B -
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Naphthalenacetic acid (NAA)
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C -
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2,4-dichlorophenoxyacetic acid
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D -
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All of the above
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2-
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Which is/are the disadvantage/(s) of using IAA in plant cell culture media?
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A -
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It is unstable in solution
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B -
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Gets easily oxidized
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C -
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Conjugated to inactive form by plant cells
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D -
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All of the above
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3-
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To maintain the pH of the culture
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A -
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organic acid such as citric, fumaric, malic and succinic acid is used
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B -
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synthetic buffers such as Tris, MES or HEPS are used
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C -
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ammonium salts are used
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D -
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Both (a) and (b)
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4-
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Which of the following is not a cytokinin?
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A -
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2,4-dichlorophenoxyacetic acid
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B -
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6 benzylaminopurine
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C -
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Zeatin
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D -
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Kinetin
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5-
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Which of the following is not an auxin?
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A -
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Indole acetic acid (IAA)
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B -
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Naphthalenacetic acid (NAA)
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C -
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zeatin
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D -
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Indole butyric acid
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6-
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Which of the following is not true about nurse or conditioned medium?
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A -
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It is liquid removed from the suspension of fast growing cells
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B -
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It contains uncharacterized growth factor released by growing cells
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C -
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It is used in the culture of regenerating protoplast
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D -
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It is removed aseptically from the culture and is autoclaved before use
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7-
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Very high sugar concentration (40-100 g/l) have been used
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A -
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in specialized secondary metabolite production
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B -
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to adjust the osmotic potential of the media in short term treatment for regeneration
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C -
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Both (a) and (b)
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D -
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None of these
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8-
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What is 'nurse' or conditioned medium?
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A -
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It is the media full of growth factors used for the growth of cells
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B -
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It is the medium added to nurse the callus culture
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C -
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It is the liquid medium removed from the suspension of fast growing cells
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D -
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Both (a) and (b)
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9-
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What are the macronutrients used in plant cell culture medium?
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A -
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N, P, K, S, Na
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B -
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N, P, K, Ca, Cl
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C -
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N, P, K, S, Ca
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D -
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N, P, Ca, Na, Cl
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10-
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Neutralized activated charcoal is occasionally added to young regenerating cultures to
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A -
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remove toxic phenolics produced by the stressed plant cell
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B -
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help to remove plants growth regulators introduced at an earlier stage
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C -
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maintain the pH of the medium
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D -
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Both (a) and (b)
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