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Biotechnology - 58

1-	Range of osmolarity tolerated/accepted in mOsm/Kg of H₂O by mammalian cells is
	A -	150-300
	B -	280-360
	C -	300-325
	D -	360-400
2-	Disaggregating of cells can be achieved by
	A -	physical disruption
	B -	enzymatic digestion
	C -	treating with chelating agents
	D -	All of the above
3-	Accumulation of lactate leads to
	A -	increase in pH
	B -	no change in pH
	C -	reduction in the pH of culture hence loss of cell viability
	D -	no loss of cell viability
4-	When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
	A -	incomplete glutamine oxidation
	B -	increase in specific lactate production from glucose
	C -	accumulation of ammonia
	D -	Both (a) and (b)
5-	According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
	A -	6 amino acids
	B -	8 amino acids
	C -	10 amino acids
	D -	12 amino acids
6-	Excess CO₂ suppress cell growth and productivity by
	A -	inhibiting respiration
	B -	altering intracellular pH by diffusing across cell membrane
	C -	altering pH of the medium
	D -	Both (a) and (b)
7-	Which of the following is incorrect?
	A -	Established cell lines (ECL) have short doubling time
	B -	ECL are invariably aneuploid
	C -	ECL grow in higher density
	D -	ECL do not show much evidence of spatial orientation
8-	What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
	A -	Lactate helps in the growth while ammonium inhibits the growth
	B -	Have no effect on cells
	C -	They act as growth inhibitors
	D -	They act as growth promoters
9-	What is the concentration of CO₂ required for culturing animal cells?
	A -	2-5%
	B -	1-10%
	C -	10-15%
	D -	15-20%
10-	Which of the following is correct?
	A -	ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
	B -	ECL and PCL can be established in suspension cultures
	C -	PCL can be established in suspension cultures
	D -	None of the above

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