Which of the followings are the metabolic products of glucose and glutamine?
A -
CO2 and NH3
B -
CO2 and lactate
C -
Lactate and ammonium
D -
Lactate only
2-
To prevent the accumulation of lactate
A -
low glutamine concentration is required
B -
high glutamine concentration is required
C -
low glucose concentration is required
D -
high glucose concentration is required
3-
What are the main constituents of culture for animal cell growth?
A -
Glucose and Glutamine
B -
Growth factors
C -
Cytokines
D -
All of these
4-
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
A -
Ethyl alcohol is being produced in excess
B -
The cells have too much oxygen
C -
Glycolysis is being inhibited
D -
The cells do not have enough oxygen
5-
Range of optimum glutamine concentration present in the culture media is
A -
1-2 mmol/litre
B -
2-7 mmol/litre
C -
7-15 mmol/litre
D -
15 - 20 mmol/litre
6-
The human fibroplast is a classical example of
A -
stable primary cell lines
B -
established cell lines
C -
cell transformations
D -
None of these
7-
pH of culture medium is initially controlled by
A -
presence of CO2
B -
presence of bicarbonate buffer
C -
addition of bases
D -
None of these
8-
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
A -
Down's syndrome
B -
Turner's syndrome
C -
Klinefelter's syndrome
D -
All of these
9-
In animal cell culture, particularly mammalian cell culture, transformation means
A -
uptake of new genetic material
B -
phenotypic modifications of cells in culture
C -
release of genetic information
D -
Both (a) and (b)
10-
How does CO2 help in the cell metabolism during cell culture?
A -
It participates in the de novo synthesis of purines and pyrimidines
