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Biotechnology - 02
Which of the following is not commonly used as vector? A. Artificial chromosome B. Cosmid C. Fungi D. Plasmid A short molecule containing 2-20 nucleotide is A. plasmid B. vector C. oligonucleotide D. mononucleotide....... Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are A. DNA ligase B. helicases C. restriction endonucleases D. DNA gyrase.... Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as A. photoreactivation B. gel electrophoresis C. autoradiography D. blotting........ Which of the following vector can maintain the largest fragment of foreign DNA? A. YAC B. Cosmid
Thời gian bắt đầu: Hôm nay lúc 20:13:29(Server time)
Số câu hỏi: 10.   Tổng điểm: 10
Thời gian làm bài: 20 phút.  Thời gian còn lại: 
Cỡ chữ câu hỏi:  Cỡ chữ đáp án:
1-
Which of the following is not commonly used as vector?
A-
Artificial chromosome
B-
Cosmid
C-
Fungi
D-
Plasmid
2-
A short molecule containing 2-20 nucleotide is
A-
plasmid
B-
vector
C-
oligonucleotide
D-
mononucleotide
3-
Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
A-
DNA ligase
B-
helicases
C-
restriction endonucleases
D-
DNA gyrase
4-
Which of the following vector can maintain the largest fragment of foreign DNA?
A-
YAC
B-
Cosmid
C-
Plasmid
D-
Phage
5-
Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
A-
photoreactivation
B-
gel electrophoresis
C-
autoradiography
D-
blotting
6-
For gene probes to be useful they must
A-
be large enough to contain gene-specific sequences
B-
be labeled in some manner to allow detection
C-
Both (a) and (b)
D-
None of the above
7-
A genomic library is
A-
a database where the sequence of an organism's genome is stored
B-
a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C-
a book that describes how to isolate DNA from a particular organism
D-
a place where the information of the genetic organization of organisms are kept.
8-
Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
A-
DNA polymerase
B-
Restriction endonuclease
C-
DNA ligase
D-
DNA helicase
9-
Bacterial cells protect their own DNA from degradation by restriction endonucleases by
A-
methylating the DNA at the sites that the enzyme recognizes
B-
deleting all recognition sites from the genome
C-
not producing any restriction endonucleases
D-
having anti restriction endonucleases
10-
Which type of restriction endonuclease cuts the DNA within the recognition site?
A-
Type I
B-
Type II
C-
Type III
D-
All of the these
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