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Biotechnology - 13
Opines that are present in crown gall tumour include A. octopine B. nopaline C. agropine D. all of these Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid? A. pRK 2013 B. pRN 3 C. either (a) or (b) Which of the following is true about T DNA? A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA....
Thời gian bắt đầu: Hôm nay lúc 23:54:55(Server time)
Số câu hỏi: 10.   Tổng điểm: 10
Thời gian làm bài: 20 phút.  Thời gian còn lại: 
Cỡ chữ câu hỏi:  Cỡ chữ đáp án:
1-
Opines that are present in crown gall tumour include
A-
octopine
B-
nopaline
C-
agropine
D-
All of these
2-
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
A-
pRK 2013
B-
pRN 3
C-
Either (a) or (b)
D-
None of these
3-
Which of the following is true about T DNA?
A-
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
B-
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
C-
Integration of T DNA occurs at two specific sites in the plant nuclear DNA
D-
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
4-
Which of the following is not true about the direct repeats flanking T-DNA?
A-
They are conserved between nopaline and octopine Ti-plasmids
B-
These repeats are transferred intact to the plant genome
C-
These are important in integration mechanism
D-
All of the above
5-
The left segment of octopine T-DNA (TL) is necessary for
A-
enzymes for agropine biosynthesis
B-
tumour formation
C-
conjugative transfer
D-
All of these
6-
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
A-
It is carried out with the help of micromanipulator
B-
The recipient cells are immobilized on artificial support or artificially bound to substarate
C-
It employs needle with diameter greater than cell diameter
D-
All of the above
7-
The right segment of octopine T-DNA (TR) is necessary for
A-
enzymes for agropine biosynthesis
B-
tumour formation
C-
conjugative transfer
D-
All of these
8-
Opine synthesis is the property
A-
conferred to plant cells when it transformed by Agrobacterium tumefaciens
B-
determined by the bacteria Agrobacterium tumefaciens
C-
Both (a) and (b)
D-
of normal plant cells
9-
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
A-
one set of sequence necessary for gene transfer
B-
two sets of sequence necessary for gene transfer
C-
three sets of sequence necessary for gene transfer
D-
four sets of sequence necessary for gene transfer
10-
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
A-
cos site
B-
flanking sequences
C-
border sequences
D-
transfer sequences
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