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Biotechnology - 39
Maximum size of foreign DNA that can be inserted into a replacement vector is A. 25-30 kb B. 18-20 kb C. 20-25 kb D. 40-50 kb View Answer Workspace Report Discuss in Forum Which of the following is not true about phagemid? A. Contain functional origin of replication of the plasmid and λ phage B. May be propagated as a plasmid or as phage in appropriate strain C. Contain λ att site D. Can only be propagated as phage View Answer Workspace Report Discuss in Forum pBR 322 has/have which of the following selection marker(s)? A. Ampr B. Tetr C. Both (a) and (b) D. Kanr....
Thời gian bắt đầu: Hôm nay lúc 22:12:20(Server time)
Số câu hỏi: 10.   Tổng điểm: 10
Thời gian làm bài: 20 phút.  Thời gian còn lại: 
Cỡ chữ câu hỏi:  Cỡ chữ đáp án:
1-
Maximum size of foreign DNA that can be inserted into a replacement vector is
A-
25-30 kb
B-
18-20 kb
C-
20-25 kb
D-
40-50 kb
2-
Which of the following is not true about phagemid?
A-
Contain functional origin of replication of the plasmid and λ phage
B-
May be propagated as a plasmid or as phage in appropriate strain
C-
Contain λ att site
D-
Can only be propagated as phage
3-
pBR 322 has/have which of the following selection marker(s)?
A-
Ampr
B-
Tetr
C-
Kanr
D-
Both (a) and (b)
4-
A plasmid can be considered as a suitable cloning vector if
A-
it can be readily isolated from the cells
B-
it possesses a single restriction site for one or more restriction enzymes
C-
insertion of foreign DNA does not alter its replication properties
D-
All of the above
5-
Difference between λ gt 10 and λ gt 11 vectors is that
A-
λ gt 11 is an expression vector
B-
λ gt 10 is an expression vector
C-
λ gt 10 is a replacement vector
D-
λ gt 11 is a replacement vector
6-
λ ZAP vector is an example of
A-
phage
B-
phagemid
C-
cosmid
D-
plasmid
7-
λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
A-
6-7 kb
B-
1-2 kb
C-
40-44 kb
D-
20-23 kb
8-
Select the wrong statement about plasmids?
A-
It is extrachromosomal
B-
It is double stranded
C-
Its replication depends upon host cell
D-
It is closed and circular DNA
9-
Stuffer is
A-
the right arm of the vector DNA
B-
the left arm of the vector DNA
C-
central fragment of the vector DNA
D-
None of the above
10-
Conjugative plasmids
A-
do not exhibit antibiotic resistance
B-
carry transfer genes called the tra genes
C-
exhibit antibiotic resistance
D-
do not carry transfer genes
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