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Biotechnology - 09

1-	When guard cells swell
	A -	stomata close
	B -	stomata open
	C -	photosynthesis stops
	D -	evaporation stops
2-	Water moves into roots of plants by
	A -	osmosis
	B -	active transport
	C -	diffusion
	D -	pressure flow mechanism
3-	A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
	A -	microshoot
	B -	medium
	C -	extant
	D -	scion
4-	Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
	A -	cellulotyic enzymes
	B -	pectolytic enzymes
	C -	both cellulotyic and pectolytic enzymes
	D -	proteolytic enzymes
5-	A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
	A -	microshoot
	B -	medium
	C -	extant
	D -	scion
6-	Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
	A -	cellulotyic enzymes
	B -	pectolytic enzymes
	C -	both cellulotyic and pectolytic enzymes
	D -	proteolytic enzymes
7-	Which method of plant propagation involves the use of girdling?
	A -	Grafting
	B -	Cuttings
	C -	Layering
	D -	Micropropagation
8-	Organogenesis is
	A -	formation of callus tissue
	B -	formation of root and shoots on callus tissue
	C -	Both (a) and (b)
	D -	genesis of organs
9-	Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
	A -	Nurse medium
	B -	Nurse or feeder culture
	C -	Both (a) and (b)
	D -	None of these
10-	In a callus culture
	A -	increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
	B -	increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
	C -	auxins and cytokinins are not required
	D -	only auxin is required for root and shoot formation

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