Which of the following is not the explantation technique?
A -
Slide culture
B -
Carrel flask culture
C -
Roller test tube culture
D -
Adherent primary culture
2-
Cells which have undergone transformation frequently become
A -
anchorage independent
B -
anchorage dependent
C -
stable
D -
unstable
3-
Range of optimum glucose concentration present in the culture media is
A -
5.5 - 55 mmol/litre
B -
55 - 75 mmol/litre
C -
75-105 mmol/litre
D -
105-150 mmol/litre
4-
The technique of organ culture may be divided on the basis of employing
A -
solid medium
B -
liquid medium
C -
semi-solid medium
D -
Both (a) and (b)
5-
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
A -
oxidative pathway
B -
anaerobic pathway
C -
Both (a) and (b)
D -
None of these
6-
An established cell line can be called where it has been subcultured at least
A -
70 times at an interval of 3 days between subcultures
B -
40 times at an interval of 3 days between subcultures
C -
70 times at an interval of 1 day between subcultures
D -
50 times at an interval of 3 days between subcultures
7-
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
A -
0.05-5 nmol of O2/cell/h
B -
5 - 10 nmol of O2 /cell/h
C -
0.05-5 pmol of O2/cell/h
D -
5 - 10 pmol of O2/cell/h
8-
Which of the following is not a source of energy in active muscle cells?
A -
Creatine phosphate
B -
ATP
C -
Lactic acid
D -
Glucose
9-
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
A -
established cell lines
B -
primary cell lines
C -
secondary cell lines
D -
propagated cell lines
10-
Higher dissolved oxygen concentration in the culture media are toxic and lead to
A -
DNA degradation
B -
lipid peroxidation
C -
metabolism of nutrients in culture media at a rate greater than that required for consumption
