Which of the following is not commonly used as vector?
A -
Artificial chromosome
B -
Cosmid
C -
Fungi
D -
Plasmid
2-
A short molecule containing 2-20 nucleotide is
A -
plasmid
B -
vector
C -
oligonucleotide
D -
mononucleotide
3-
Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
A -
DNA ligase
B -
helicases
C -
restriction endonucleases
D -
DNA gyrase
4-
Which of the following vector can maintain the largest fragment of foreign DNA?
A -
YAC
B -
Cosmid
C -
Plasmid
D -
Phage
5-
Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
A -
photoreactivation
B -
gel electrophoresis
C -
autoradiography
D -
blotting
6-
For gene probes to be useful they must
A -
be large enough to contain gene-specific sequences
B -
be labeled in some manner to allow detection
C -
Both (a) and (b)
D -
None of the above
7-
A genomic library is
A -
a database where the sequence of an organism's genome is stored
B -
a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C -
a book that describes how to isolate DNA from a particular organism
D -
a place where the information of the genetic organization of organisms are kept.
8-
Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
A -
DNA polymerase
B -
Restriction endonuclease
C -
DNA ligase
D -
DNA helicase
9-
Bacterial cells protect their own DNA from degradation by restriction endonucleases by
A -
methylating the DNA at the sites that the enzyme recognizes
B -
deleting all recognition sites from the genome
C -
not producing any restriction endonucleases
D -
having anti restriction endonucleases
10-
Which type of restriction endonuclease cuts the DNA within the recognition site?
